"They can make an appeal to the League if they want to try and get the ban lifted and it has been known to work – Notts County were successful last season – but they must prove to us that they cannot field a competitive side due to injuries," Nagle said.
Barnsley's injury problems have been well documented - 11 players were out of action when the Reds took on Port Vale last Saturday - but Nagle stressed "If they have got senior players who are capable of playing then it is unlikely that they will get special dispensation."
The news will come as a big blow to Tykes boss Glyn Hodges, who recently revealed that he has been in talks with three ex-Premiership players.