The Reds reserves lost 2-1 to a Blades side containing former keeper Sasa Ilic.
Reserves beaten by the Blades
The blades opened the scoring on 59 through Jon Forte and extended their lead six minutes later as with a Wayne Allison header.
Griff Jones gave the Reds hope on 81 minutes but it was not enough.
Lee Crooks and Brian O'Callaghan made comebacks after injury.
Lee Crooks and Brian O'Callaghan made comebacks after injury.
Barnsley Line-up: Flinders, Tonge, Williams (Atkinson 61), Handyside, O'Callaghan (Laight 70), Crooks (Heslop 66), Lumsdon, Wroe, Jones, Jarman, Baker.