Reserves result: Barnsley 2 Tranmere 1
On 28 minutes the Reds won a penalty after Daniel Nardiello's header was saved on the line by defender Neil Ashton who was sent-off but Nardiello's penalty was saved.
Nardiello did put the Reds in front on 35 minutes from a Nathan Jarman pass but Tranmere were level terms early in the second half from a Simon Haworth shot.
Barnsley were back in front after 57 minutes through a Nathan Jarman header.
BARNSLEY LINE-UP: Scarsella, Harban, Farmery, Laight, Atkinson, Burns, Heslop, Jarman, Onibuje (Scothern 80), Nardiello (Joynes 45), Baker.
Subs: Flinders, Beevor, Batley.