A 92nd minute penalty denied Barnsley the chance to make the FA Cup Fourth Round for the first time since 2012-2013.
It also means that since beating Chelsea in the 2007-2008 Quarter-Final, the Reds have lost their last five FA Cup matches against Premier League opposition by an aggregate score of 2-15. (Burnley 0-1, Manchester City 0-5, Swansea 2-4, West Ham 0-2, West Ham 0-3)
However this was the first one of those five where Barnsley went in at half-time level.
It is the second time in a row that Barnsley have been level against a Premier League team at half-time after also being goal-less with Tottenham in the League Cup last season.
This was the first time Barnsley have lost a match in injury time since the 23rd September 2017 when a 93rd minute Alfred N'Diaye strike secured a 2-1 win for Wolves at Molineux.
VAR was used in a Barnsley game for the first time ever and it reversed a first half penalty decision initially given for a foul by Dimi Cavaré on Madej Vydra however it was overturned as Vydra was offside. It is though the first time Barnsley have conceded two penalties in one game since August 2016 against QPR.
Remarkably Burnley were awarded two penalties in one match having gone 64 games without being awarded one last doing so in the League Cup in September 2017 - this was also converted late on by Chris Wood (89th) and against a Yorkshire opponent (Leeds)
Wood who had a fairly forgettable loan spell at Oakwell in 2010 - failing to score in seven appearances - has scored in his last two appearances against the Reds, including netting a double for Leeds at Oakwell in January 2017 - with one also being a penalty.
Wood has now scored four goals in the third round for four different teams - West Brom, Brighton, Leicester and Burnley.
Each of the last three meetings with Burnley have now finished 1-0 with the Clarets winning all three.
Four of the last six have also finished with this scoreline and that includes Barnsley winning an FA Cup Third Round tie in 2013.
No team has scored more than once in any of the last six meetings with Burnley being the last team to do so in 2012.
There was 11,053 in attendance at Turf Moor - the lowest crowd at the ground for a FA Cup tie since January 2015 (9,348) - surprisingly the opponents were Tottenham.
Ryan Hedges made only his fifth start of the season and the first since November.
Barnsley have gone back to back games without scoring for the first time this season and the first time since December 2017.
It is the first time they have failed to score in their first two games of a calendar year since 2009 when they failed to score in their first three.
The Reds have now won just one of their last 11 visits to face Burnley at Turf Moor in all competitions (D2 L8), winning 2-1 in the Championship in December 2008.
Burnley have now lost just one of their last nine home FA Cup games (W7 D1)
This is the tenth time a Barnsley match has been goal-less at half time this season
Despite playing a Premier League side Barnsley enjoyed the majority of the ball possession (52%) - for the 32nd time this season
Burnley had 11 shots, the most by an opponent since Bristol Rovers also had 11 in October, however only two of those were on target including the penalty winner.
Barnsley themselves had nine efforts, but failed to manage a single shot on target for the first time this season.
Barnsley Team:
1. Adam Davies (c)
12. Dimitri Cavaré
5. Ethan Pinnock
6. Liam Lindsay
28. Ben Williams
8. Cameron McGeehan
7. Ryan Hedges
27. Alex Mowatt
21 Mike-Steven Bähre
26. Mamadou Thiam
25. Cauley Woodrow
Substitutes:
19. Kieffer Moore (for Hedges 63)
10. George Moncur (for Thiam 77)
4. Kenny Dougall (for Bähre 83)
Unused Substitutes:
18. Adam Jackson, 29. Victor Adeboeyjo, 33. Jacob Brown, 35. Jake Greatorex
Burnley Team:
29. Nick Pope
5. James Tarkowski (c)
28. Kevin Long
14. Ben Gibson
23. Stephen Ward
31. Dwight McNeil
16. Steven Defour
13. Jeff Hendrick
3. Charlie Taylor
27. Matej Vydra
9. Sam Vokes.
Substitutions:
7. Johann Berg Gudmundsson (for Ward 59)
11. Chris Wood (for Vokes 74)
4. Jack Cork (for Defour 78)
Unused Substitutes:
6. Ben Mee, 10. Ashley Barnes, 18. Ashley Westwood, 20. Joe Hart.
Burnley manager Sean Dyche said:
"We've pieced together a side and I'm pleased because that was a good Barnsley side.
"I felt we had enough to win the game and did enough to win the game, especially at the end of a big, important week for us.
"I'm a big advocate of VAR but the referee needs to hold the ball. I know they want to be right, but they do need to speed it up and those things will get ironed out.
"The referee allowed the set-up for a penalty and I know we are learning about it, but the referee should grab the ball and walk outside the penalty area, so there are no implications.
"Matej had got to the point of his run-up before the referee blew his whistle and said 'no'.
"That delay was what annoyed me - not the outcome - because once you know that is right you quickly move on."
Match Reports:
FA Cup Third Round results:
Friday 4th January 2019:
Tranmere Rovers 0-7 Tottenham Hotspur
Aurier 40
Llorente 48
Aurier 55
Son Heung-Min 57
Llorente 71
Llorente 72
Kane 82
Saturday 5th January 2019:
AFC Bournemouth 1-3 Brighton & Hove Albion
Knoackaert 31
Bissouma 34
Pugh 55
Andone 64
Burnley 1-0 BARNSLEY
Wood pen 90+2
Manchester United 2-0 Reading
Mata pen 22
Lukaku 45+4
Sheffield Wednesday 0-0 Luton Town
Shrewsbury Town 1-1 Stoke City
Norburn pen 45+4
Crouch 78
West Bromwich Albion 1-0 Wigan Athletic
Sako 31
West Ham United 2-0 Birmingham City
Arnautovic 2
Carroll 90+1
Accrington Stanley 1-0 Ipswich Town
Kee 76
Aston Villa 0-3 Swansea City
Baker-Richardson 2
Dyer 47
Fulton 78
Bolton Wanderers 5-2 Walsall
Cook 19
Donaldson 58
Magennis 61
Guthrie og 63
Beevers og 68
Magennis 80
Magennis 87
Brentford 1-0 Oxford United
Maupay pen 80
Chelsea 2-0 Nottingham Forest
Morata 49
Morata 59
Derby County 2-2 Southampton
Redmond 4
Redmond 48
Marriott 58
Lawrence 61
Everton 2-1 Lincoln City
Lookman 12
Bernard 14
Bostwick 28
Fleetwood Town 2-3 AFC Wimbledon
Barcham 16
Hartigan 55
Madden 70
Evans pen 72
Appiah 90
Gillingham 1-0 Cardiff City
List 81
Middlesbrough 5-0 Peterborough United
Assombalonga 47
Friend 50
Wing 62
Assombalonga 70
Fletcher 87
Blackpool 0-3 Arsenal
Willock 11
Willock 37
Iwobi 82
Bristol City 1-0 Huddersfield Town
Brownhill 72
Crystal Palace 1-0 Grimsby Town
Ayew 86
Newcastle United 1-1 Blackburn Rovers
Dack 56
Ritchie pen 84
Norwich City 0-1 Portsmouth
Green 90+4
League One results:
Charlton Athletic 1-1 Sunderland
O'Nien 2
James og 50
Rochdale 0-4 Burton Albion
Myers-Harness 19
Myers-Harness 30
Boyce 78
Myers-Harness 86
Scunthorpe United 2-1 Coventry City
Perch 67
Sutton 7
Chaplin 78
Sunday 5th January 2019:
Fulham 1-2 Oldham Athletic
Odoi 52
Surridge pen 76
Lang 88
Manchester City 7-0 Rotherham United
Sterling 12
Foden 43
Ajayi og 45+1
Gabriel 52
Mahrez 73
Otamendi 78
Sane 85
Millwall 2-1 Hull City
Toral 52
Ferguson 82
Ferguson 85
Preston North End 1-3 Doncaster Rovers
Marquis 5
Hughes 56
Anderson 72
Wilks 87
Queens Park Rangers 2-1 Leeds United
Oteh pen 23
Halme 25
Bidwell 75
Sheffield United 0-1 Barnet
Coulthirst pen 21
Woking 0-2 Watford
Hughes 13
Deeney 74
Newport County 2-1 Leicester City
Matt 10
Ghezzal 82
Amond pen 85
Monday 6th January 2019:
Wolverhampton Wanderers 2-1 Liverpool
Jiménez 38
Origi 51
Neves 55
Barnsley drop to 5th place in Sky Bet League One with 46 points from 25 games. We are 2 points inside the Play-Offs, 6 points off the Automatic Promotion Places, 11 points off First Place and 19 points above the Relegation Zone.
Up to 06/01/19
Next Up:
At Oakwell:
Sky Bet League One Matchday Twenty Six: Barnsley v Bradford City, Saturday 12th January 2019. Kick-Off: 3pm
On The Road:
Sky Bet League One Matchday Twenty Seven: AFC Wimbledon v Barnsley, Saturday 19th January 2019. Kick-Off: 3pm